Quercus spinosa Raw sequence reads. Quercus spinosa

we report a modified method for the isolation of intact chloroplasts, which needs less than 0.5 g leaf material. Coupled with rolling circle amplification (RCA), next-generation sequencing and a pipeline combining de-novo assembly and reference guided assembly (RGA), we successfully obtained a complete chloroplast genome for the non-model forest tree species: evergreen oak Quercus spinosa, with as many as 36% of the sequence reads mapped to the chloroplast genome.

本研究报道了一种改良的完整叶绿体分离方法，该方法仅需不到0.5克的叶片材料。结合滚环扩增（RCA）、下一代测序技术，以及整合从头组装（de-novo assembly）与参考基因组引导组装（RGA）的分析流程，我们成功获取了非模式林木树种刺叶栎（*Quercus spinosa*，常绿栎类）的完整叶绿体基因组，其测序读段中多达36%可比对至该叶绿体基因组。