Gene expression profile of p16-induced senescence in normal human diploid fibroblasts.

Transcriptional profiling of p16-induced senescent human diploid fibroblasts compared with proliferating cells. TIG-3 ER-p16 cells (primary normal human diploid fibroblasts expressing a 4-hydroxytamoxifen(4-OHT) regulatable form of human p16) were cultured for 7 days with or without 4-OHT. Total RNA was isolated using TRIzol reagent and were analyzed using the hum

与增殖细胞相比，p16诱导衰老的人二倍体成纤维细胞的转录组分析。TIG-3 ER-p16细胞（表达经4-羟基他莫昔芬（4-hydroxytamoxifen，缩写4-OHT）调控的人p16的原代正常二倍体成纤维细胞）在添加或不添加4-OHT的条件下培养7天。使用TRIzol试剂提取总RNA，并采用人类（原文此处内容未完整）进行分析。