LINCS MCF 10A Common Project: Fixed-time-point sensitivity measures of the MCF 10A breast cell line to 8 small molecule perturbagens. Dataset 9 of 9: Calculated dose-response metrics for biological replicate 3

To generate measures of the sensitivities of three independent batches and one derivative of the MCF 10A cell line to 8 small molecule perturbagens, we treated cells with single drugs over a minimum 9-point dilution series using SQRT(10) dilutions centered around the GR50 but not exceeding 10 µM and then measured cell number after three days of drug exposure.

为获取MCF 10A细胞系（MCF 10A cell line）的3个独立批次及1株衍生株对8种小分子扰动剂的敏感性量化指标，我们采用以50%生长抑制浓度（GR50）为中心、最高浓度不超过10 μM、以SQRT(10)为稀释梯度的至少9点浓度稀释体系，以单一药物处理细胞，并于药物暴露3天后检测细胞数量。