raw data for“Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz)”

 Cassava (Manihot esculenta Crantz) efficiently accumulates starch in its storage roots. However, how photosynthates are transported from the leaves to the phloem (especially how they are unloaded into parenchymal cells of storage roots) remains unclear. Results: Here, we investigated the sucrose unloading pattern and its impact on cassava storage root development using microstructural and physiological analyses, namely, carboxyfluorescein (CF) and C14 isotope tracing. The expression profiling of genes involved in symplastic and apoplastic transport was performed, which included enzyme activity, protein gel blot analysis, and transcriptome sequencing analyses. These finding showed that carbohydrates are transported mainly in the form of sucrose, and more than 54.6% was present in the stem phloem. Sucrose was predominantly unloaded symplastically from the phloem into storage roots;

木薯（Manihot esculenta Crantz）能有效地在其储存根中积累淀粉。然而，光合产物从叶片向筛管（尤其是它们如何卸载到储存根的薄壁细胞中）的运输过程尚不明确。研究结果：在本研究中，我们利用微结构和生理分析方法，即羧基荧光素（CF）和C14同位素示踪，对木薯储存根中蔗糖的卸载模式及其对储存根发育的影响进行了研究。我们进行了参与质体和细胞间隙运输的基因表达谱分析，包括酶活性、蛋白凝胶电泳分析和转录组测序分析。这些发现表明，碳水化合物主要以蔗糖的形式运输，其中超过54.6%存在于茎的筛管中。蔗糖主要从筛管通过质体途径卸载到储存根中。