MicroRNA profiling of primary high-grade soft tissue sarcomas

This screening revealed unique miRNA expression patterns for synovial sarcomas , myxoid liposarcomas, and leiomyosarcomas, and defined unique sets of miRNAs discriminating the different liposarcoma subtypes from non-neoplastic adipose tissue. We performed a comprehensive miRNA expression analysis in a series of 76 untreated, primary high-grade soft tissue sarcomas representing eight subtypes, and in 15 sarcoma cell lines using microarray technology. Additionally, two normal fat samples were analysed.

本筛查明确了滑膜肉瘤、黏液样脂肪肉瘤和平滑肌肉瘤的独特微小RNA（microRNA, miRNA）表达谱，并确定了可将不同脂肪肉瘤亚型与非肿瘤性脂肪组织区分开的特异性miRNA集合。本研究采用微阵列技术，对涵盖8种亚型的76例未接受治疗的原发性高级别软组织肉瘤、15株肉瘤细胞系开展了全面的miRNA表达谱分析；此外还分析了2份正常脂肪组织样本。