How a paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody

Paramyxoviruses including measles, Nipah, and parainfluenza viruses are public health threats with pandemic potential. Human parainfluenza virus type 3 (HPIV3) is a leading cause of illness in pediatric, elderly, and immunocompromised populations. There are no approved vaccines or therapeutics for HPIV3. Neutralizing antibodies that target viral fusion are a potential strategy for mitigating paramyxovirus infection, however their utility may be curtailed by viral evolution that leads to resistance. Paramyxoviruses enter cells by fusing with the cell membrane in a process mediated by a complex consisting of a receptor binding protein (HN) and a fusion protein (F). Existing atomic resolution structures fail to reveal physiologically relevant interactions during viral entry. We present cryo-ET structures of pre-fusion HN-F complexes in situ on the surfaces of authentic virions that evolved resistance to an anti-HPIV3 F neutralizing mAb. Single mutations in F abolis..., , , # Data from: How a paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody

[https://doi.org/10.5061/dryad.g1jwstqz6](https://doi.org/10.5061/dryad.g1jwstqz6)

Excel file including the raw data for the article \"How a Paramyxovirus Fusion/Entry Complex Adapts to Escape a Neutralizing Antibody.\"

## Description of the data and file structure

We have submitted our raw data for figures 1,2,3, and 5 (Hats_Off_Paper_Raw_Data_Final.xlsx), plasmid sequences encoding HN, HA, and F (Sequences.xlsx), and uncropped western blot surface expressions of F variants using anti-HPIV3 F HRC

### Descriptions

**Hats_Off_Paper_Raw_Data_Final.xlsx**

In Hats_Off_Paper_Raw_Data_Final.xlsx each tab contains the raw data and is labelled with the figure panels used in the manuscript: How a Paramyxovirus fusion/entry complex adapts to escape a neutralizing antibody.

·      HN: hemagglutinin-neuraminidase protein

·      F: Fusion protein

·      HA: Uncleaved influenza hemagglutinin

·     ...,

副粘病毒科病毒（Paramyxoviruses）涵盖麻疹病毒、尼帕病毒及副流感病毒，均为具备大流行潜力的公共卫生威胁。人3型副流感病毒（Human parainfluenza virus type 3, HPIV3）是儿童、老年人与免疫功能低下人群发病的首要诱因，目前尚无获批的HPIV3疫苗或治疗药物。以病毒融合过程为靶点的中和抗体是缓解副粘病毒感染的潜在策略，但病毒进化引发的耐药性可能会限制其应用效果。副粘病毒通过与宿主细胞膜融合侵入细胞，该过程由受体结合蛋白（HN）与融合蛋白（F）构成的复合物介导。现有原子分辨率结构无法揭示病毒侵入过程中生理相关的相互作用。本研究报道了在针对抗HPIV3 F中和单克隆抗体（mAb）产生耐药性的天然病毒粒子表面原位存在的融合前状态HN-F复合物的冷冻电镜断层扫描（cryo-ET）结构。F蛋白的单点突变可使... , , , # 数据来自：《副粘病毒融合/侵入复合物如何逃逸中和抗体》
https://doi.org/10.5061/dryad.g1jwstqz6
本文《副粘病毒融合/侵入复合物如何逃逸中和抗体》的原始数据存储于如下Excel文件中。
## 数据与文件结构说明
本研究提交了图1、2、3及5的原始数据（文件名为Hats_Off_Paper_Raw_Data_Final.xlsx）、编码HN、HA与F蛋白的质粒序列（文件名为Sequences.xlsx），以及使用抗HPIV3 F HRC抗体检测F变体表面表达的未裁剪免疫印迹原始数据。
### 文件说明
#### Hats_Off_Paper_Raw_Data_Final.xlsx
该文件的每个工作表均存储有对应原始数据，工作表标签对应本文《副粘病毒融合/侵入复合物如何逃逸中和抗体》中的图版：
· HN：血凝素-神经氨酸酶蛋白（hemagglutinin-neuraminidase protein）
· F：融合蛋白（Fusion protein）
· HA：未切割的流感血凝素（Uncleaved influenza hemagglutinin）
· ...