Origin-by-QTL interaction analysis in the (DAxPVG) G10 population.

The statistical confirmation of the parent-of-origin dependent QTLs identified in the backcross populations. Analysis was performed using the fit-multiple QTL model. A full model comprised all QTLs and Parent-of-origin (G9) x QTL interactions (Phenotype ∼ QTL1a + QTL1a*ORIGIN + QTL3 + QTL3*ORIGIN + QTL4a + QTL4a*ORIGIN + QTL5b + QTL5b*ORIGIN + QTL7b + QTL7b*ORIGIN + QTL10a + QTL10a*ORIGIN + QTL10c + QTL10c*ORIGIN + QTL14 + QTL14*ORIGIN + ε). In the next stage the effect of each QTL or origin x QTL interaction was subtracted from the full model and the contribution of the subtracted term to the full model was evaluated and expressed in p-values. Analysis was performed in 794 G10 rats comprising 366 malesa and 428 femalesb. n/a, analysis could not be applied due to lack of evidence for a QTL.对回交群体中鉴定到的亲本起源依赖型数量性状位点（Quantitative Trait Locus, QTL）进行统计验证。本分析采用拟合多重QTL模型开展。全模型包含所有QTL以及亲本来源（G9）与QTL的交互效应，模型表达式为：表型~QTL1a + QTL1a*ORIGIN + QTL3 + QTL3*ORIGIN + QTL4a + QTL4a*ORIGIN + QTL5b + QTL5b*ORIGIN + QTL7b + QTL7b*ORIGIN + QTL10a + QTL10a*ORIGIN + QTL10c + QTL10c*ORIGIN + QTL14 + QTL14*ORIGIN + ε。后续分析阶段，我们从全模型中逐一移除单个QTL或亲本来源-QTL交互效应项，并评估该移除项对全模型的贡献，以p值形式呈现评估结果。本次分析共纳入794只G10大鼠，其中雄性366只（标注a）、雌性428只（标注b）。无可用数据：因未检测到QTL相关证据，无法开展分析。