Golden Hamster genome sequencing project. Mesocricetus auratus

We sequenced and assembled the Golden hamster genome using PacBio Sequel. To obtain a chromosome-scale genome assembly, we aligned all contigs to the 22 golden hamster chromosomes (MesAur1.0_HiC, DNA Zoo). We attempted to fill 3,797 gaps in the reference chromosomes using the assembled contigs and error-corrected reads. The nucleotide difference between the DNA Zoo MesAur1.0_HiC assembly and our assembly was 0.140%.本研究采用PacBio Sequel测序平台完成金黄仓鼠基因组的测序与组装。为获得染色体级基因组组装结果，我们将所有重叠群（contig）比对至22条金黄仓鼠染色体参考序列（MesAur1.0_HiC，DNA Zoo）。本研究尝试利用组装得到的重叠群与经错误校正的测序读段（reads），填补参考染色体中的3797个基因组缺口。DNA Zoo发布的MesAur1.0_HiC组装版本与本研究组装版本之间的核苷酸差异率为0.140%。