Overall gene expression comparison between antimony resistant and sensitive Leishmania donovani

The mRNA expression of antimony resistant strains of Leishmania donovani was compared to the expression of the sensitive Leishmania donovani. The antimony resistant and sensitive Leishmania donovani were grown in complete M199 medium with 10% FCS and Penicillin streptomycin mixture. At stationary phase (5 day culture) cells were harvested in sterile Phosphate buffered saline and used for RNA isolation.

本数据集对比了杜氏利什曼虫（Leishmania donovani）锑抗性菌株与敏感菌株的mRNA表达水平。两类菌株均接种于添加10%胎牛血清（Fetal Calf Serum，FCS）与青霉素-链霉素混合液的完全M199培养基（M199 medium）中培养。待细胞培养至静止期（即培养第5天）时，采用无菌磷酸盐缓冲液（Phosphate Buffered Saline，PBS）收集细胞，并将其用于RNA提取。