Mouse lung tissue transcriptome response to influenza H5N1 and mutants [mRNA](Agilent-028005). Mus musculus

The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse lung infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203') and VN1203-NS1trunc124. Overall design: Groups of 22-week-old C57BL/6 mice were infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203') and VN1203-NS1trunc124 (an NS1 protein with a 90 amino acid truncation at the C-terminus). Infections were done at 10^4 PFU/mouse in 50 μl PBS. Time points were 1, 2, 4, and 7 d.p.i. There were 5 mice for all infection groups and 3 mice for all mock groups. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

本数据集旨在获取感染野生型A/Vietnam/1203/2004（H5N1；简称‘VN1203’）与VN1203-NS1trunc124（该毒株的NS1蛋白C端存在90个氨基酸截短）的小鼠肺部样本，用于mRNA、微小RNA（microRNA, miRNA）、蛋白质组学、脂质组学、代谢组学及组织病理学分析。实验设计：选取22周龄C57BL/6小鼠，以每只小鼠10^4 蚀斑形成单位（plaque forming unit, PFU）的病毒量，溶于50μL磷酸盐缓冲液（phosphate buffered saline, PBS）中进行感染。采样时间点为感染后1、2、4、7天（days post infection, d.p.i.）。所有感染组均设置5只小鼠，空白对照组（mock组）设置3只小鼠。收集肺部样本用于病毒载量检测、转录组与蛋白质组学分析；同时监测小鼠的体重减轻情况及存活率。