Characterization of the N- and C-terminal domain interface of the three main apoE isoforms

Chemical cross-linking coupled to mass spectrometry was used to investigate the conformations of the apolipoprotein isoforms apoE2, apoE3, and apoE4. Cross-linking data was generated using the reagents disuccinimidyl suberate (DSS), pimelic dihydrazide (PDH), and 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride. Intramolecular interactions derived from the cross-linking experiments were combined with molecular modeling approaches.

本研究采用化学交联结合质谱分析法（Chemical cross-linking coupled to mass spectrometry），探究载脂蛋白亚型（apolipoprotein isoforms）apoE2、apoE3及apoE4的构象。实验通过辛二酰二亚胺（disuccinimidyl suberate，DSS）、庚二酰二酰肼（pimelic dihydrazide，PDH）以及4-(4,6-二甲氧基-1,3,5-三嗪-2-基)-4-甲基吗啉鎓氯化物（4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride，DMTMM）三种交联试剂获取了交联数据。将交联实验得到的分子内相互作用数据与分子建模方法相结合。