RNA-seq from mice CD4+CD25+ splenocytes

To better understand the function of CD4+CD25+ splenocytes from different ages, target cells from young(8-week old) aged(18-month old) were sorted with magnetic beads. We then performed gene expression profiling analysis using data obtained from RNA-seq of different cells from different age. Overall design: Comparative gene expression profiling analysis of RNA-seq data for CD4+CD25+ splenocytes of aged and young mice sorted with magnetic beads.

为更清晰地解析不同年龄小鼠CD4+CD25+脾细胞的功能，我们采用磁珠分选法分离了8周龄年轻小鼠与18月龄老年小鼠的靶细胞。随后，我们基于不同年龄来源的各类细胞的RNA测序（RNA-seq）数据，开展了基因表达谱分析。整体实验设计：对经磁珠分选得到的年轻与老年小鼠CD4+CD25+脾细胞的RNA测序数据进行对比基因表达谱分析。