Molecular pathways and putative transcription regulatory networks affected during cardiac aging.

Overview of differential gene expression analysis between 10 days and 40 days adult cardiac tubes. Enrichment p values were calculated using a test based on hypergeometric distribution.
(1)Statistically significantly deregulated genes between 10 and 40 day-old dissected hearts were determined using Limma software from normalized expression data (See Table S1 and S2).
(2)GO term enrichments were determined using the Flymine database (www.flymine.org) (see Table S5 for details).
(3)[13]; See Table S6 for details.
(4)[14]; See Table S7 for details.
(5)[17], [18]; see Table S9 for details.
(6)Transcriptional regulatory network were predicted with cisTargetX (http://med.kuleuven.be/cme-mg/lng/cisTargetX/.) The z score of the most representative TF-binding motif is indicated for each motif family. (See Table S8 for details).

本数据集为10日龄与40日龄成体心管的差异基因表达分析概览。富集p值基于超几何分布检验计算得到。
(1) 利用Limma软件对标准化表达数据进行分析，筛选出10日龄与40日龄解剖心脏间具有统计学显著性的差异表达基因（详见补充表S1与S2）。
(2) 采用Flymine数据库（www.flymine.org）完成基因本体（Gene Ontology, GO）功能富集分析，详细信息见补充表S5。
(3) 参考文献[13]，详细信息见补充表S6。
(4) 参考文献[14]，详细信息见补充表S7。
(5) 参考文献[17]、[18]，详细信息见补充表S9。
(6) 借助cisTargetX（http://med.kuleuven.be/cme-mg/lng/cisTargetX/）预测转录调控网络，为每个基序家族标注其最具代表性的转录因子（Transcription Factor, TF）结合基序的z得分，详细信息见补充表S8。