Transcriptome analysis of brain endothelial cells from adult EC-specific Glut1 knock-out mice as compared to wild type controls

To explore the mechanisms through which GLUT1 controls EC function, we compared transcriptomic alterations induced by loss of GLUT1 by performing RNA sequencing analysis on freshly isolated ECs from adult (8 week old) GLUT1EC-/- brains. In adult brains ECs, 1494 genes were differentially expressed between WT and GLUT1-/- ECs but we did not observe compensatory upregulation of other GLUTs. Interestingly, pathway analysis revealed that ECs from GLUT1EC-/- mice were characterized by a robust activation of inflammatory and extracellular matrix pathways. Examination of transcriptomic differences in GLUT1EC-/- vs WT controls

为探究葡萄糖转运蛋白1（Glucose Transporter 1, GLUT1）调控内皮细胞（Endothelial Cell, EC）功能的分子机制，我们对成年（8周龄）GLUT1EC基因敲除小鼠脑组织中新鲜分离的内皮细胞开展RNA测序分析，对比GLUT1缺失诱导的转录组学变化。在成年小鼠脑组织内皮细胞中，野生型（Wild Type, WT）与GLUT1基因敲除内皮细胞间共鉴定出1494个差异表达基因，但未观察到其他葡萄糖转运蛋白家族成员出现代偿性上调。值得注意的是，通路分析结果显示，GLUT1EC基因敲除小鼠的内皮细胞呈现出炎症通路与细胞外基质（extracellular matrix）通路的显著激活特征。针对GLUT1EC基因敲除组与野生型对照组的转录组差异分析