Tumor T4 Sectors. Homo sapiens

The following CGH experiments were conducted on four sectors (S1-S4) from a single primary ductal carcinoma tumor (T4). Overall design: The genomic DNA from each tumor sector was labeled with Cy5 and hybridized to an 85K Bgl2 ROMA Microarray. A normal reference male fibroblast was labeled with Cy3 as a control. Samples were dye-swapped and the experiments were conducted in color-reversal. The value data repesents a log ratio of the mean of two dye-swapped samples

本研究针对单发性原发性导管癌（primary ductal carcinoma）肿瘤（T4）的四个分区（S1-S4）开展了比较基因组杂交（Comparative Genomic Hybridization, CGH）实验。实验设计概况：将各肿瘤分区的基因组DNA以Cy5标记并与85K Bgl2 ROMA微阵列芯片进行杂交；以雄性成纤维细胞作为正常参照样本，并用Cy3标记作为对照。所有样本均进行染料互换处理，并以颜色反转模式完成实验。所得数值数据为两份染料互换样本均值的对数比值（log ratio）。