Expression data for drought-, cold, high-salinity and ABA treatment in Arabidopsis plants

We applied the tiling arrays to study the Arabidopsis whole-genome transcriptome under drought, cold, high-salinity and ABA treatment conditions and idenfied many stress- or ABA- responsive putative functional RNAs and fully-overlapping sense-antisense transcripts in Arabidopsis genome. Keywords: stress response Two-week-old Arabidopsis plants grown on the agar plates were subjected to the stress- or ABA- treatments. The total RNA was prepared from the treated- and untreated- plants, and used for the microarray hybridization. Three replicative hybridization experiments for each strand array were carried out using the independent biological RNA samples.

本研究采用平铺式基因芯片（tiling array），对干旱、低温、高盐及脱落酸（ABA）处理条件下的拟南芥（Arabidopsis）全基因组转录组展开分析，在拟南芥基因组中鉴定出多种响应胁迫或脱落酸的推定功能RNA，以及完全重叠的正义-反义转录本。关键词：胁迫响应。将培养于琼脂平板的两周龄拟南芥植株施以胁迫或脱落酸处理，分别从处理组与未处理组植株中提取总RNA，用于微阵列杂交实验。针对每种链特异性阵列芯片（strand array），采用独立的生物学重复RNA样本开展三次重复杂交实验。