Transcriptome analysis of VapC21 overexpression and deletion in Mycobacterium tuberculosis.. Transcriptome analysis of VapC21 overexpression and deletion in Mycobacterium tuberculosis.

We report the effects of VapC21 overexpression on Mycobacterium tuberculosis H37Rv strain. The total RNA was isolated from M. tuberculosis harboring either pTetR-int or pTetR-Int-vapC21 the expression was induced with 50 ng/ml anhydrotetracycline for 24 hrs. We report the effect of deletion VapC21 on the transciptional profile of Mycobacterium tuberculosis Erdman strain. For RNA-seq analysis, total RNA was isolated from mid-log phase culture of either parental or △vapC21 mutant strain. Overall design: Experiments were repeated thrice for each overexpression strain and twice for each deletion strain.

本研究报道了VapC21过表达对结核分枝杆菌（Mycobacterium tuberculosis）H37Rv菌株的影响。实验分别从携带pTetR-int或pTetR-Int-vapC21的结核分枝杆菌中提取总RNA，以50 ng/ml脱水四环素诱导表达24小时。本研究同时报道了VapC21基因缺失对结核分枝杆菌Erdman菌株转录谱的影响。针对RNA测序（RNA-seq）分析，总RNA分别从亲本菌株或△vapC21突变菌株的中期对数生长期培养物中提取。实验设计：每株过表达菌株的实验均重复3次，每株缺失菌株的实验均重复2次。