Histone Modification in Cerebral Arteriovenous Malformation

Cerebral arteriovenous malformations (AVMs) are the most common vascular malformations worldwide and the leading cause of hemorrhagic strokes that result in crippling neurological deficits. Here, using newly generated mouse model, we discovered that genome-wide ocuppancy of H4K8ac and H3K27me3 decreased in mouse cerebral AVMs. Overall design: Chromatin immunoprecipitation followed by DNA sequencing (ChIP-seq) to discover histone modification of H4K8ac and H3K27me3 in mouse model of cerebral arteriovenous malformations.

脑动静脉畸形（Cerebral arteriovenous malformations, AVMs）是全球范围内最常见的血管畸形，亦是引发致残性神经功能缺损的出血性脑卒中的首要病因。本研究利用新构建的小鼠模型，发现小鼠脑动静脉畸形组织中，全基因组范围内H4K8ac与H3K27me3的结合占有率均出现下调。整体实验设计：采用染色质免疫共沉淀联合DNA测序（Chromatin immunoprecipitation followed by DNA sequencing, ChIP-seq）技术，分析小鼠脑动静脉畸形模型中H4K8ac与H3K27me3的组蛋白修饰特征。