Utilizing allele-specific expression for the study of wound healing in MRL mice

MRL mice have the unique ability to regenerate ear punch wounds, while dorsal wounds heal with a scar, enabling controlled comparisons of regenerative and non-regenerative repair mechanisms in the same mouse. Using hybrid crosses between MRL and non-regenerating mice (CAST/Eij), we capitalize on allele-specific expression to identify cis- regulatory variation associated with divergent ear and dorsal healing outcomes.

MRL小鼠（MRL mice）具有独特的耳穿刺伤口再生能力，而其背部伤口愈合后会形成瘢痕，这使得在同一只小鼠中即可对再生性与非再生性修复机制开展可控的比较研究。本研究通过MRL小鼠与非再生型小鼠（CAST/Eij）的杂交后代，借助等位基因特异性表达技术，筛选与耳部和背部伤口愈合结局差异相关的顺式调控变异。