Expression profile of Pseudomonas putida KT2440 growing in myristic acid. Expression profile of Pseudomonas putida KT2440 growing in myristic acid

We report the application of a high-throughput technique, RNA-seq, to study the transcriptomic pattern of P. putida KT2440 cultures using myristic acid as the sole carbon source at the exponential phase of growth compared with cultures grown with glucose as the sole carbon source. Thus, we can explore the expression of genes involved in fatty acid degradation. Overall design: P. putida KT2440 mRNA profiles growing in myristic acid or glucose as sole carbon source

本研究报道了高通量技术RNA测序（RNA-seq）的应用，用于对比分析以肉豆蔻酸为唯一碳源、处于指数生长阶段的恶臭假单胞菌（P. putida KT2440）培养物，与以葡萄糖为唯一碳源培养的该菌培养物的转录组图谱。借此可解析参与脂肪酸降解的基因表达情况。实验设计：以肉豆蔻酸或葡萄糖作为唯一碳源培养的恶臭假单胞菌（P. putida KT2440）的mRNA转录组图谱。