H3K4me2-Abcam.Kc

modENCODE_submission_4935 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: Kc167; Tissue: embryo-derived cell-line; Developmental Stage: late embryonic stage; Genotype: se/e; Sex: Female; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line Kc167; Antibody H3K4me2-Abcam (target is H3K4me2)

modENCODE_submission_4935 本提交源自Gary Karpen主导的modENCODE项目。如需查阅modENCODE项目完整列表，请访问http://www.genome.gov/26524648。项目目标：本研究旨在确定黑腹果蝇（Drosophila melanogaster）基因组中主要组蛋白修饰的定位位点。所研究的组蛋白修饰参与DNA复制、基因表达、基因沉默以及遗传等核心染色体功能。我们将采用染色质免疫沉淀（ChIP）结合基因组平铺阵列开展实验。初步将利用三种细胞系与两个胚胎阶段的染色质进行定位分析，后续将针对部分蛋白质的分析拓展至另外四种动物组织/发育阶段。关于数据使用的条款与细则，请参阅http://www.genome.gov/27528022 及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。实验类型：ChIP-chip。生物来源：细胞系：Kc167；组织类型：胚胎来源细胞系；发育阶段：晚期胚胎阶段；基因型：se/e；性别：雌性；重复次数：4；实验因子：Kc167细胞系；抗体：H3K4me2-Abcam（靶标为H3K4me2）