Expression data from mice lung CD45-negative cells during fibrosis development

We used microarrays to detail the global gene expression change and identified distinct classes of up-regulated genes during fibrosis development in lung non-hematopoietic cells. Close interaction between nonhematopoietic and hematopoietic cells are critical for fibrosis development. Total RNA was isolated from mouse lung CD45-negative cells 10 days after PBS or BLM inoculation using an RNA isolation kit and subjected to microarray analysis

本研究通过基因芯片（microarray）解析了纤维化进程中的全基因组基因表达变化，并鉴定出肺非造血细胞在纤维化发展阶段的不同类别上调基因。非造血细胞与造血细胞之间的紧密相互作用对于纤维化进程至关重要。研究人员从经磷酸盐缓冲液（PBS）或博来霉素（BLM）接种10天后的小鼠肺CD45阴性细胞中提取总RNA，采用RNA提取试剂盒完成提取后，对样本进行基因芯片分析。