Toxicity effect of five insecticides on the development and enzyme activities of Trichogramma ostriniae

As an egg parasitoid, Trichogramma ostriniae (T. ostriniae) exhibits a broad host range and plays a crucial role in controlling various lepidopteran agricultural pests. However, the application of chemical pesticides negatively impacts its development and survival. Therefore, it is essential to assess the toxicity of commonly used insecticides against T. ostriniae and evaluate their compatibility. This study aims to to determine the toxic effects of five common insecticides (dinotefuran, abamectin, imidacloprid, beta-cypermethrin, and chlorantraniliprole) on the development, reproduction, and enzymatic activity of Trichogramma ostriniae. The contact, lethal, and developmental toxicities were evaluated. Activities of detoxification enzymes, including cytochrome P450 (CYP450), carboxylesterase (CarE), and glutathione S-transferase (GST), and protective enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) as well as malondialdehyde (MDA) and reactive oxygen speci..., Corcyra cephalonica (Stainton) (C. cephalonica) were used as hosts to establish a stable population of T. ostriniae by breeding multiple generations. The feeding conditions were 25°C, 75% relative humidity, and a 15 L: 9D photoperiod.The diluted insecticide was evenly distributed on the inner wall of a glass tube (54.6 cm2) to construct the insecticide membrane. For the contact test, a total of 100 ± 10 adult T. ostriniae that had emerged for 6 h were put into each tube containing the insecticide membrane after all the acetone was volatilized and fed with 10% honey water. After being sealed with a black cloth, the tubes were placed for 8 h. The number of dead and surviving adult T. ostriniae in the tube was counted.The safety evaluation of pesticides on T. ostriniae can be divided into four levels according to the safety factor: low risk, moderate risk, high risk, extreme high risk. Safety factor = LR50/recommended dose in the field.Egg parasitism was achieved by exposing T. ostriniae t..., , # Toxicity effect of five insecticides on the development and enzyme activities of Trichogramma ostriniae

## Description of the data and variables:

Figure 1A presents the raw data for activity of CYP450.

Figure 1B presents the raw data for activity of CarE.

Figure 1C presents the raw data for activity of GST.

Figure 2A presents the raw data for activity of SOD.

Figure 2B presents the raw data for activity of POD.

Figure 2C presents the raw data for activity of CAT.

Figure 3A presents the raw data for MDA content.

Figure 3B presents the raw data for ROS content.

Figure 4A presents the raw data for MRCC1 content.

Figure 4B presents the raw data for ATP content.

Table 2 provides the raw data on the toxicity of selected pesticides to adult T. ostriniae.

Table 3 provides the raw data on the risk evaluation of selected pesticides to adult* T. ostriniae*.

Table 4 provides the raw data on the emergence rate of adult T. ostriniae exposed to insecticides at different developmental ...

作为一种卵寄生蜂，亚洲玉米螟赤眼蜂（Trichogramma ostriniae，以下简称T. ostriniae）寄主范围广泛，在防控多种鳞翅目农业害虫中发挥着关键作用。然而，化学农药的施用会对其生长发育与存活产生负面影响。因此，评估常用杀虫剂对T. ostriniae的毒性并评价其兼容性至关重要。本研究旨在明确5种常用杀虫剂——噻虫嗪（dinotefuran）、阿维菌素（abamectin）、吡虫啉（imidacloprid）、高效氯氟氰菊酯（beta-cypermethrin）和氯虫苯甲酰胺（chlorantraniliprole）——对T. ostriniae的生长发育、繁殖及酶活性的毒性效应。研究评价了其触杀毒性、致死毒性与发育毒性，并检测了细胞色素P450（CYP450）、羧酸酯酶（CarE）、谷胱甘肽S-转移酶（GST）等解毒酶，以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）等保护酶的活性，同时检测了丙二醛（MDA）与活性氧（ROS）等指标。本研究以米蛾（Corcyra cephalonica (Stainton)，以下简称C. cephalonica）为寄主，通过多代繁育建立稳定的T. ostriniae种群。饲养条件设置为温度25℃、相对湿度75%、光周期15L:9D。将稀释后的杀虫剂均匀涂布于54.6 cm²的玻璃试管内壁，制备成药剂膜。触杀试验中，待丙酮完全挥发后，将100±10头羽化6 h的T. ostriniae成虫接入含药剂膜的试管中，以10%蜂蜜水饲喂，用黑布密封试管后放置8 h，随后统计试管内成虫的存活与死亡数量。农药对T. ostriniae的安全性评价可根据安全系数划分为4个等级：低风险、中等风险、高风险、极高风险，其中安全系数=LR50/田间推荐使用剂量。通过将T. ostriniae……以实现卵寄生。# 五种杀虫剂对亚洲玉米螟赤眼蜂生长发育及酶活性的毒性效应

## 数据与变量说明：
图1A展示了细胞色素P450（CYP450）活性的原始数据。
图1B展示了羧酸酯酶（CarE）活性的原始数据。
图1C展示了谷胱甘肽S-转移酶（GST）活性的原始数据。
图2A展示了超氧化物歧化酶（SOD）活性的原始数据。
图2B展示了过氧化物酶（POD）活性的原始数据。
图2C展示了过氧化氢酶（CAT）活性的原始数据。
图3A展示了丙二醛（MDA）含量的原始数据。
图3B展示了活性氧（ROS）含量的原始数据。
图4A展示了MRCC1含量的原始数据。
图4B展示了ATP含量的原始数据。
表2提供了供试杀虫剂对T. ostriniae成虫毒性的原始数据。
表3提供了供试杀虫剂对T. ostriniae成虫风险评价的原始数据。
表4提供了不同发育阶段暴露于杀虫剂的T. ostriniae成虫羽化率的原始数据。