Graminella nigrifrons Transcriptome or Gene expression

Insects are the most important epidemiological factors of plant virus disease spread, with >75% of viruses dependent on insects for host transmission. However, little is known regarding the molecular and cellular mechanisms that regulate the transmission processes and determine the efficiency of transmission. The black-faced leafhopper (Graminella nigrifrons) vectors two viruses which utilize different strategies for host transmission: the semi-persistent Maize chlorotic dwarf virus and the persistent propagative Maize fine streak virus. To identify genes that could be involved in a common or differential response to virus exposure, gene expression profiles were compared between G. nigrifrons fed on MCDV-infected, MFSV-infected, or healthy maize for four hours or seven days.昆虫是植物病毒病害传播的首要流行病学相关因素，超过75%的植物病毒依赖昆虫完成寄主间传播。然而，目前对于调控病毒传播过程、决定传播效率的分子与细胞机制仍知之甚少。黑面叶蝉（Graminella nigrifrons）是两种病毒的传毒媒介，这两种病毒采用不同的寄主传播策略：半持久性传播的玉米褪绿矮缩病毒（Maize chlorotic dwarf virus, MCDV）以及持久性增殖型传播的玉米细条纹病毒（Maize fine streak virus, MFSV）。为筛选出可能参与病毒暴露后的共通或差异化应答的基因，本研究对取食感染MCDV、感染MFSV或健康玉米的黑面叶蝉，分别在取食4小时及7天后的基因表达谱进行了对比分析。