Low cost transcriptomic mapping of TMT associated neurotoxicity using human minibrains

RNA sequencing was performed on minibrains exposed to distinct doses and treatment exposures of TMT using BRB sequencing. Overall design: We collected transcriptome of minibrains (derived from human induced neural stem cells derived from iPScs) exposed to 0uM, 1uM, 2.5uM and 5uM for 1 day, 3 days and 1 week.

本研究采用BRB测序技术，对暴露于不同剂量及处理时长的串联质量标签（TMT）的迷你脑（minibrain）进行了RNA测序。实验整体设计：我们采集了由源自诱导多能干细胞（iPSCs）的人诱导神经干细胞构建的迷你脑的转录组数据，该类迷你脑分别经0μM、1μM、2.5μM及5μM的TMT处理，并分别于处理1天、3天及1周后完成取样。