Next Generation Sequencing assesses 53BP1-regulated gene expression profiling in hTRET-RPE1 cells

The goal of this experiment is to analyze global gene expression profiles after depletion of 53BP1 hTERT-RPE1 53BP1 knock-out cell line used in this study were generated using CRISPR-Cas9 gene editing approach. Cell were transduced with a lentiviral expression vector encoding a single guide RNAs (gRNAs) against 53BP1, and cells expressing the vector containing only Cas9, but no sgRNA, served as control cell lines.

本实验旨在分析53BP1基因敲除后的全基因组表达谱。本研究使用的hTERT-RPE1 53BP1敲除细胞系采用CRISPR-Cas9基因编辑技术构建：将靶向53BP1的单向导RNA（single guide RNAs，gRNAs）编码序列克隆至慢病毒表达载体，并用该载体转导上述细胞；同时设置仅转导携带Cas9但不含单向导RNA（sgRNA）的载体的细胞作为对照细胞系。