Analysis of proline and lysine post-translational modifications of collagen from prolyl 3-hydroxylase 3 and lysyl hydroxylase 1 null mice

We performed shotgun proteomic analysis of type V collagens purified from wild-type mice and prolyl 3-hydroxylase 3 or lysyl hydroxylase 1 null mice by LC-MS after trypsin digestion for identification of lysine glycosylation sites.

我们对从野生型小鼠以及脯氨酰3-羟化酶3（prolyl 3-hydroxylase 3）或赖氨酰羟化酶1（lysyl hydroxylase 1）基因敲除小鼠中纯化得到的V型胶原蛋白开展了鸟枪蛋白质组学分析：样品先经胰蛋白酶酶解，再通过液相色谱-质谱联用（LC-MS）技术鉴定其中的赖氨酸糖基化位点。