Whole-genome nucleosome mapping in meiotic diploid S. cerevisiae

We determined nucleosome positions throughout the genome in diploid S. cerevisiae undergoing early stages of synchronous meiosis. This study sought to assess if systematic reorganization of nucleosomes occurs during meiotic prophase at or near sites of DNA double strand break formation. Overall design: Six samples were sequenced, each a separate time point from either of two meiotic time courses. Dataset N1 consists of samples collected at 0, 1, 2, and 3 hr in meiosis; Dataset N2 consists of samples collected at 0 and 4 hr. Time courses, sample preparation, and sequencing were carried out in separate laboratories for the two datasets: Hochwagen lab (Whitehead Institute) for Dataset N1; Keeney lab (Howard Hughes Medical Institute; Memorial Sloan-Kettering Cancer Center) for Dataset N2.

本研究解析了处于同步减数分裂早期阶段的二倍体酿酒酵母（S. cerevisiae）全基因组范围内的核小体（nucleosome）定位情况。本研究旨在探究减数分裂前期中，DNA双链断裂形成位点及其邻近区域是否发生核小体的系统性重排布。实验设计概述：共对6个样本进行测序，所有样本均来自两套独立的减数分裂时间进程实验，每个样本对应一个独立的时间节点。数据集N1包含减数分裂0、1、2及3小时采集的样本；数据集N2包含减数分裂0及4小时采集的样本。两套数据集的时间进程实验、样本制备及测序工作均在不同实验室完成：数据集N1的相关实验由霍克瓦根实验室（怀特黑德研究所）完成；数据集N2的相关实验由基尼实验室（霍华德·休斯医学研究所；纪念斯隆·凯特琳癌症中心）完成。