Genome sequence of the H2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant

Abstract We report on the nearly complete genome sequence of Clostridium beijerinckii strain Br21, formerly isolated from a sugarcarne vinasse wastewater treatment plant. The resulting genome is ca. 5.9 Mbp in length and resembles the size of previously published C. beijerinckii genomes. We annotated the genome sequence and predicted a total of 5323 genes. Strain Br21 has a genetic toolkit that allows it to exploit diverse sugars that are often found after lignocellulosic biomass pretreatment to yield products of commercial interest. Besides the whole set of genes encoding for enzymes underlying hydrogen production, the genome of the new strain includes genes that enable carbon sources conversion into butanol, ethanol, acetic acid, butyric acid, and the chemical block 1,3-propanediol, which is used to obtain polymers. Moreover, the genome of strain Br21 has a higher number of ORFs with predicted beta-glucosidase activity as compared to other C. beijerinckii strains described in the KEGG database. These characteristics make C. beijerinckii strain Br21 a remarkable candidate for direct use in biotechnological processes and attest that it is a potential biocatalyst supplier.

摘要 本研究报道了此前从糖制酒糟废水处理厂分离得到的拜氏梭菌（Clostridium beijerinckii）菌株Br21的近乎完整基因组序列。该基因组全长约5.9 Mbp，与已发表的拜氏梭菌基因组大小相近。本研究对该基因组序列进行了注释，共预测得到5323个基因。菌株Br21携带一套遗传工具箱，能够利用木质纤维素生物质预处理后常见的多种糖类，合成具有商业价值的产物。除编码产氢相关酶的全套基因外，该新菌株的基因组还包含可将碳源转化为丁醇、乙醇、乙酸、丁酸以及用于制备聚合物的化工结构单元1,3-丙二醇的基因。此外，相较于KEGG数据库中收录的其他拜氏梭菌菌株，Br21菌株基因组中预测具有β-葡萄糖苷酶活性的开放阅读框（Open Reading Frame, ORF）数量更多。上述特性使拜氏梭菌Br21成为直接应用于生物技术工艺的优质候选菌株，同时证实其是极具潜力的生物催化剂供应源。