EGFR defificiency leads to impaired self-renewal and pluripotency of mouse embryonic stem cells

By using the AG1478 inhibition model and RNA-seq analysis, we discovered the importance of EGFR in self-renewal and pluripotency through regulating cell cycle and the expression of pluripotency factors. As a cell surface receptor conveying exogenous stimulations, EGFR deficiency led to cell cycle arrest, and impaired self-renewal and pluripotency. These findings will improve our understanding of the molecular mechanisms driving the self-renewal and pluripotency of mESCs.

本研究通过构建AG1478抑制模型并开展RNA测序（RNA-seq）分析，明确了表皮生长因子受体（EGFR）通过调控细胞周期及多能性因子表达，在干细胞自我更新与多能性维持中的核心作用。作为介导外源刺激的细胞表面受体，EGFR缺失会引发细胞周期阻滞，进而损害自我更新能力与多能性。上述研究结果将深化我们对驱动小鼠胚胎干细胞（mESCs）自我更新与多能性的分子机制的认知。