HiRIEF-LC-MS based proteomics analysis of subcellular fractions of human CD4+ T-Cells with and without T-cell Receptor stimulation.

Subcellular proteomics analysis of human T-cells in response to T-cell receptor stimulation. High Resolution Isoelectric Focusing (HiRIEF) LC-MS/MS and relative quantification by TMT 10-plex was used to analyze subcellular fractions (Cytosol, Nuclear and Mitochondria-Small Organelle-Membrane) of Human T-cells from 3 separate donors stimulated with T-cell receptor stimulation for 0, 15 minutes or 60 minutes. The data was obtained from 3 separate TMT10 plex sets which included: 126 – Cytosol, unstimulated; 127N – Cyto, 15 min; 127C – Cyto, 60 min; 128N – Mitochondrion-Small Organelle-Membrane, unstimulated; 128C – Mito-SmO-Mem, 15 min; 129N – Mito-SmO-Mem, 60 min; 129C – Nuclear, unstimulated; 130N – Nuc, 15 min; 130C– Nuc, 60 min; 131 – pooled internal control of all samples.

针对T细胞受体刺激的人类T细胞的亚细胞蛋白质组学分析。本研究采用高分辨率等电聚焦液相色谱-串联质谱（High Resolution Isoelectric Focusing, HiRIEF LC-MS/MS）与TMT 10-plex相对定量技术，对3名独立供体的人类T细胞的亚细胞组分（胞质、细胞核及线粒体-小细胞器-膜组分）进行分析，供体细胞分别经T细胞受体刺激0、15分钟或60分钟。该数据集来源于3组独立的TMT 10-plex实验套装，各组标记对应信息如下：126——未刺激的胞质组分；127N——刺激15分钟的胞质组分；127C——刺激60分钟的胞质组分；128N——未刺激的线粒体-小细胞器-膜组分；128C——刺激15分钟的线粒体-小细胞器-膜组分；129N——刺激60分钟的线粒体-小细胞器-膜组分；129C——未刺激的细胞核组分；130N——刺激15分钟的细胞核组分；130C——刺激60分钟的细胞核组分；131——所有样本的混合内参。