Genome-wide study of NF-YC11 regulatory network in rice

Rice NF-YC11 is a transcription factor that plays a key regulatory role in storage substance accumulation during rice grain filling. To reveal the transcription regulatory network of NF-YC11 in rice, we performed genome-wide identification of NF-YC11 targets by immunoprecipitation sequencing (ChIP-seq) analyses in the NF-YC11-overexpression plants. Genome-wide identification binding sites of NF-YC11 in rice endosperm. Examination of 7 days after pollination (DAP) NF-YC11-FLAG rice endosperm with FLAG antibody and Input seres as control.

水稻NF-YC11是一种转录因子，在水稻籽粒灌浆期的贮藏物质积累过程中发挥关键调控作用。为解析水稻NF-YC11的转录调控网络，本研究针对NF-YC11过表达植株开展免疫沉淀测序（immunoprecipitation sequencing，ChIP-seq）分析，实现全基因组水平的NF-YC11靶标鉴定，并完成了水稻胚乳中NF-YC11的全基因组结合位点鉴定。本研究以授粉后7天（days after pollination，简称DAP）的NF-YC11-FLAG水稻胚乳为实验材料，采用FLAG抗体进行检测，并以Input样本作为对照。