Inhibition of γ-glutamyl transpeptidase suppresses LPS induced asthma exacerbation in a mouse model. Inhibition of γ-glutamyl transpeptidase suppresses LPS induced asthma exacerbation in a mouse model

To investigate the role of γ-glutamyltranspeptidase in severe asthma, we established an OVA/ LPS-induced asthma model. Inflammation analysis and second-generation sequencing revealed the mechanism by which inhibition of γ-glutamyltranspeptidase inhibited LPS-induced asthma exacerbation in mice. Overall design: In order to study the effect of GGsTop on asthma, we set up control group (PBS), asthma group (OL), and experimental group (OLG), and isolated trachea for transcriptomic sequencing. Gene expression profiling was performed on RNA-seq data.

为探究γ-谷氨酰转肽酶（γ-glutamyltranspeptidase）在重症哮喘中的作用，我们构建了卵清蛋白（OVA）/脂多糖（LPS）诱导的哮喘模型。通过炎症分析与二代测序（second-generation sequencing），我们揭示了γ-谷氨酰转肽酶抑制可缓解小鼠体内脂多糖诱导的哮喘急性加重的作用机制。实验设计概述：为探究GGsTop对哮喘的干预效果，我们设置了对照组（PBS）、哮喘组（OL）以及实验组（OLG），分离气管组织进行转录组测序，并基于RNA测序（RNA-seq）数据开展基因表达谱分析。