Genes regulated by CREB1 in LPS-primed THP1 monocytes

To screen the genes and pathways regulated by CREB1 in the THP1 monocytes induced by LPS, THP1 monocytes were transfected by CREB1 shRNA and then treated by LPS for 3 hours. Comparative gene expression profiling analysis of RNA-seq data for THP1 monocytes and CREB1 shRNA with or without LPS treatment..

为筛选脂多糖（Lipopolysaccharide, LPS）诱导的THP1单核细胞（THP1 monocytes）中受环腺苷酸应答元件结合蛋白1（CREB1）调控的基因与通路，本研究将THP1单核细胞经CREB1短发夹RNA（shRNA）转染后，用LPS处理3小时。随后对经或未经LPS处理的THP1单核细胞，以及转染CREB1 shRNA并经或未经LPS处理的THP1单核细胞的RNA测序（RNA-seq）数据开展比较基因表达谱分析。