Table_2_Initial Sequencing and Characterization of the Gastrointestinal and Oral Microbiota in Urban Pakistani Adults.XLSX

We report the initial characterization of the gastrointestinal tract (gut) and oral microbiota (bacteria) in 32 urban Pakistani adults. Study participants were between ages 18 and 40, had body mass index between 18 and 25 Kg/m2, and were students or early-career professionals. These individuals donated a total of 61 samples (32 gut and 29 oral) that were subjected to 16S ribosomal RNA (rRNA) gene sequencing. Microbiome composition of Pakistani individuals was compared against the uBiome database of selected individuals who self-reported to be in excellent health. We observed strong gender-based differences in the gut microbiome of Pakistani individuals, a skewness toward Firmicutes, and unusually high levels of Proteobacteria in the Pakistani men. These observations may indicate microbiota dysbiosis, though 16S data alone can neither establish cause nor effect to human health. Albeit conducted on a smaller scale, our report provides a first snapshot about the composition and diversity of gut and oral microbiota communities in Pakistani individuals.

本研究首次对32名巴基斯坦城市成年人的胃肠道（gastrointestinal tract，下称gut）与口腔微生物群（细菌）开展表征分析。受试者年龄介于18至40岁，身体质量指数（body mass index，BMI）处于18~25 kg/m²区间，职业为学生或职场初期从业者。上述受试者共计捐献61份样本（其中32份为肠道样本、29份为口腔样本），所有样本均完成16S核糖体RNA（16S ribosomal RNA，下称16S rRNA）基因测序。本研究将巴基斯坦受试者的微生物组组成，与uBiome数据库中自我报告健康状况极佳的筛选受试者数据进行对比分析。结果显示，巴基斯坦受试者的肠道微生物组存在显著性别差异：菌群组成偏向厚壁菌门（Firmicutes），且巴基斯坦男性体内变形菌门（Proteobacteria）的丰度异常偏高。上述发现或提示存在微生物群失调，但仅依靠16S测序数据无法明确其与人类健康之间的因果关系。尽管本研究规模较小，本报告仍首次展现了巴基斯坦人群肠道与口腔微生物群群落的组成及多样性特征。