LC-MS/MS data from Neofusicoccum parvum strains grown in YMG medium with or without grapevine cane powder

Neofusicoccum parvum is a wood-colonizing fungus capable of infecting numerous economically important crops. In particular, this fungal trunk pathogen represents a significant threat to worldwide grape production. Neofusicoccum parvum’s ability to colonize and damage the host may rely on the secretion of diverse effectors, such as phytotoxic specialized metabolites. Despite the scientific efforts to discover and understand the role of specialized metabolites in disease development, there is a lack of information on the factors influencing the phytotoxin production in N. parvum and how this pathogen modulates its metabolome when facing the host. In this study, we evaluated the specialized metabolites production of two N. parvum wild-type strains (Bt67 and NpB) and a UV mutant (UV9) cultured in 20-L bioreactors for six days. To give insight into how N. parvum adapts the specialized metabolite production in contact with host tissue, the three strains were cultured in the presence and absence of grapevine cane powder. Daily ethyl acetate extracts were analyzed using HPLC-MS/MS. This dataset corresponds to the mzML files generated from the culture filtrates of the three strains grown with or without grapevine cane powder.

小新壳梭孢菌（Neofusicoccum parvum）是一种木质定殖真菌，可侵染多种具有重要经济价值的作物。作为林木枝干病原菌，该菌对全球葡萄生产构成重大威胁。小新壳梭孢菌定殖宿主并造成病害的能力，可能依赖于其分泌的多种效应因子（effector），例如植物毒性特化代谢物（specialized metabolites）。尽管学界已开展诸多研究以探明特化代谢物在病害发生发展中的作用，但目前仍缺乏关于影响小新壳梭孢菌植物毒素（phytotoxin）合成的相关因子，以及该病原菌在接触宿主时如何调控自身代谢组（metabolome）的相关信息。

本研究针对两株小新壳梭孢菌野生型菌株（wild-type strains，Bt67与NpB）以及一株紫外线诱变突变株（UV9）展开实验：将三株菌株分别置于20升生物反应器（bioreactors）中培养6天，对其特化代谢物的合成情况进行评估。为揭示小新壳梭孢菌在接触宿主组织时如何调控特化代谢物的合成，实验设置了添加葡萄藤茎粉（grapevine cane powder）与不添加葡萄藤茎粉两组培养条件。每日收集乙酸乙酯（ethyl acetate）萃取物，并采用高效液相色谱-串联质谱（HPLC-MS/MS）进行分析。

本数据集对应于上述三株菌株在添加/不添加葡萄藤茎粉的培养条件下，其培养液滤液经质谱分析生成的mzML格式文件。