Gene expression profiles of MCF7 with CARM1 knocked down

The goal of this study is to identify ERalpha-target genes affected by knocking down of the histone arginine methyltransferase CARM1 in MCF7 breast cancer cells. The roles of CARM1 in ERalpha+ breast cancer was not well characterized. Therefore, we created a Dox inducible CARM1 knockingdown MCF7 cell line where CARM1 is decreased to 20% of endogeneous level to determine the created a Dox-inducible CARM1shRNA overexpressing MCF7 cells for evaluation of the global effects of CARM1 on ERalpha-target gene expression. MCF7-tet-on-CARM1shRNA clone 1 were treated under 4 conditions: DMSO; Dox; E2 (10nM); Dox+E2. In Dox+E2 condition, cells were pre-treated with Dox for 5 days before treating with E2 for 4 hours. 3 biological replicates were included and total of 12 samples were analyzed.

本研究旨在明确MCF7乳腺癌细胞中，组蛋白精氨酸甲基转移酶CARM1（histone arginine methyltransferase CARM1）敲低所影响的雌激素受体α（ERalpha）靶基因。此前，CARM1在ERalpha阳性乳腺癌中的作用尚未得到充分表征。因此，我们构建了多西环素（Dox）诱导型CARM1敲低MCF7细胞系，将CARM1表达量降至内源性水平的20%；同时构建了过表达CARM1短发夹RNA（CARM1shRNA）的MCF7细胞系，以全面评估CARM1对ERalpha靶基因表达的全局调控效应。将MCF7-tet-on-CARM1shRNA克隆1号分为4组进行处理：二甲基亚砜（DMSO）对照组、多西环素（Dox）处理组、雌二醇（E2，10nM）处理组以及Dox+E2联合处理组。其中Dox+E2联合处理组的细胞需先经Dox预处理5天，再加入E2处理4小时。本实验设置3次生物学重复，共计12份样本用于后续分析。