Epigenetic Activation and alteration of chromatin structure regulated by NSD2 in metastatic castration-resistant prostate cancer (ChIP-seq)

To elucidate the regulation of NSD2 in metastatic castration-resistant prostate cancer(CRPC), we performed ChIP-seq of H3K36me2, H3K27me3, H3K4me1, H3K4me3,H3K27ac and NSD2 against castration-sensitive prostate cancer cell line LNCaP and metastatic castration-resistant prostate cancer cell lines, PC3 and DU145, respectively. In metastatic CRPC, we found specific regions of activation with epigenetic changes. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for prostate cancer cell lines

为阐明NSD2在转移性去势抵抗性前列腺癌（castration-resistant prostate cancer, CRPC）中的调控机制，我们分别对去势敏感前列腺癌细胞系LNCaP，以及转移性去势抵抗性前列腺癌细胞系PC3、DU145，开展了针对H3K36me2、H3K27me3、H3K4me1、H3K4me3、H3K27ac及NSD2的染色质免疫共沉淀DNA测序（Chromatin immunoprecipitation DNA-sequencing, ChIP-seq）。在转移性CRPC中，我们发现了伴随表观遗传改变的特异性激活区域。本数据集对应前列腺癌细胞系的染色质免疫共沉淀DNA测序（ChIP-seq）实验。