Testing a new method for the quantification of exopolymeric substances in the waters (TEP and CSP)

Transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP) represent distinct groups of microbial exopolymer particles (EPS) that play a role in e.g. oil dispersion and MOS formation. We used the well-established spectrophotometrical methods as well as a recently published method that allows direct observations of TEP and CSP in whole water samples using a portable flow-through microscope (FlowCAM). Water samples were taken at different depths at three sites (Taylor Energy, OC26, and GC767) onboard R/V Point Sur (PS17-05).

透明胞外聚合物颗粒（Transparent exopolymer particles, TEP）与考马斯亮蓝可染颗粒（Coomassie stainable particles, CSP）是两类截然不同的微生物胞外聚合物颗粒（microbial exopolymer particles, EPS），它们可在石油分散、MOS形成等过程中发挥作用。本研究采用了成熟的分光光度法，以及一种近期发表的可借助便携式流通式显微镜（FlowCAM）对全水样中的TEP与CSP进行直接观测的方法。研究团队搭乘"瑟尔角号"科考船（R/V Point Sur，航次PS17-05），在三个采样位点（泰勒能源、OC26、GC767）的不同深度采集了水样。