Gene expression analysis to find molecular mechanisms underlying the role of miR-342-5p in HER2 breast cancer. Gene expression analysis to find molecular mechanisms underlying the role of miR-342-5p in HER2 breast cancer

The molecular mechanisms behind the miR-342-5p-induced HER2 pathway regulation are not known. Here, we aimed to further investigate the role of miR-342-5p in HER2 driven breast cancer cells. Transfected cells were incubated for 24 hours, and total RNA were run on mRNA arrays. Overall design: Two HER2 amplified breast cancer cell lines were transiently transfected with miR-342-5p mimic or scrambled negative control miRNA. Transfections were done in three separate biological replicates.

miR-342-5p诱导人类表皮生长因子受体2（Human Epidermal Growth Factor Receptor 2，HER2）通路调控的分子机制目前尚未明确。本研究旨在进一步探究miR-342-5p在HER2驱动型乳腺癌细胞中的生物学功能。将转染后的细胞孵育24小时后，提取总RNA并开展mRNA芯片（mRNA array）检测。实验整体设计如下：选取两株HER2基因扩增型乳腺癌细胞系，分别瞬时转染miR-342-5p模拟物与随机序列阴性对照miRNA，每组设置三次独立生物学重复。