Detection of colonization capacity of probiotic Bifidobacterium breve CCFM1025in the human gut - Supplementary Table

Aim: To detect the gut colonization capacity of Bifidobacterium breve CCFM1025 with clinical

antidepressant-like effects. Materials & methods: A unique gene sequence of B. breve CCFM1025 was discovered based on the genome analysis of 104 B. breve strains and a strain-specific primer (1025T5) was designed. In vitro and in vivo samples were used to validate the specificity and quantitative capability of this primer in the PCR system. Results: Quantitative PCR using strain-specific primers enabled absolute quantification of CCFM1025 in fecal samples within 104–1010 cells/g (R2 >0.99). CCFM1025 remained highly detectable in volunteer feces 14 days after cessation of administration, demonstrating its favorable colonization characteristics. Conclusion: CCFM1025 can colonize the healthy human gut.

研究目的：探究具备临床类抗抑郁功效的短双歧杆菌（Bifidobacterium breve）CCFM1025的肠道定植能力。
材料与方法：本研究基于104株短双歧杆菌的基因组分析，鉴定出菌株CCFM1025的特异性基因序列，并设计了菌株特异性引物（1025T5）。通过体外与体内样本验证该引物在聚合酶链式反应（PCR）体系中的特异性与定量性能。
结果：采用菌株特异性引物的定量PCR可在10⁴~10¹⁰ 细胞/克粪便样本中对CCFM1025实现绝对定量，决定系数R²>0.99。在受试志愿者停止摄入该菌株14天后，仍可在其粪便样本中高效检出CCFM1025，证实其具备优良的肠道定植特性。
结论：短双歧杆菌CCFM1025可在健康人体肠道内定植。