RNA-seq transcriptomic data of Pseudomonas aeruginosa PAO1 and bacteriophage vB_Pae_QDWS

This dataset contains RNA-seq transcriptomic data of Pseudomonas aeruginosa PAO1 during infection by the lytic bacteriophage vB_Pae_QDWS. Samples were collected at four time points post-infection: 0, 3, 6, and 20 minutes, in order to investigate the dynamic transcriptional response of the host bacterium to phage infection. Total RNA was extracted, ribosomal RNA was removed, and strand-specific libraries were prepared and sequenced using the Illumina platform. Clean reads were mapped to the reference genomes of both P. aeruginosa PAO1 and phage vB_Pae_QDWS. This time-resolved transcriptomic dataset provides insights into the molecular interactions between bacteriophage and host, and may serve as a valuable resource for understanding phage-host dynamics, bacterial defense mechanisms, and the regulation of phage replication.

本数据集包含裂解性噬菌体vB_Pae_QDWS感染铜绿假单胞菌（Pseudomonas aeruginosa）PAO1过程中的RNA测序（RNA-seq）转录组数据。为探究宿主细菌对噬菌体感染的动态转录响应，研究人员在感染后0、3、6及20分钟四个时间点采集样本。实验中提取总RNA并去除核糖体RNA（ribosomal RNA），构建链特异性文库，随后采用Illumina测序平台完成测序。将所得洁净读段（clean reads）比对至铜绿假单胞菌PAO1与噬菌体vB_Pae_QDWS的参考基因组。该时间分辨率转录组数据集可为解析噬菌体与宿主间的分子互作提供新见解，同时可作为研究噬菌体-宿主动态互作、细菌防御机制及噬菌体复制调控的宝贵资源。