From Code to Life: Transforming Computer Algorithms into DNA

After obtaining the desired sequence, DNA synthesis is essential to produce the H-Bot in physical form. This process can be efficiently managed through a closed control loop system. The system comprises four vials containing dNTPs: deoxyadenosine triphosphate (dATP), deoxyguanosine triphosphate (dGTP), deoxythymidine triphosphate (dTTP), and deoxycytidine triphosphate (dCTP). Each vial is equipped with a micropipette for precise handling.
During synthesis, the first nitrogenous base displayed on screen triggers a robotic arm attached to a CDS micropipette to dispense a specified concentration of the corresponding dNTP. This process repeats until 23 separate DNA strands are synthesized. The resulting physical DNA strand is termed "Artificial Single-Stranded DNA" or "assDNA" strand for short. Read more ...
Garg, R. , Vyas, A. , Khan, A. , Tariq, M. (2024), 'Codes beyond Bits and Bytes: A Blueprint for Artificial Life', World Academy of Science, Engineering and Technology, Open Science Index 213, International Journal of Biotechnology and Bioengineering, 18(9), 114 - 126.

在获取目标序列后，DNA合成是将H-Bot实体化的必要环节。该流程可通过闭环控制系统实现高效管控。此系统包含四个装有脱氧核糖核苷三磷酸（dNTPs）的试剂瓶，分别为脱氧腺苷三磷酸（deoxyadenosine triphosphate，dATP）、脱氧鸟苷三磷酸（deoxyguanosine triphosphate，dGTP）、脱氧胸苷三磷酸（deoxythymidine triphosphate，dTTP）以及脱氧胞苷三磷酸（deoxycytidine triphosphate，dCTP）。每个试剂瓶均配备微量移液器以实现精准操作。
合成过程中，屏幕上显示的首个含氮碱基将触发连接于CDS微量移液器的机械臂，释放指定浓度的对应脱氧核糖核苷三磷酸。该流程循环往复，直至合成23条独立的DNA链。最终得到的实体DNA链被称为“人工单链DNA”，简称“assDNA链”。更多详情请见……
Garg, R.、Vyas, A.、Khan, A.、Tariq, M. (2024), 《超越比特与字节的编码：人工生命蓝图》，世界科学、工程与技术学院，开放科学索引213，《国际生物技术与生物工程期刊》，18(9)，114-126。