16S rRNA sequencing reveals an altered composition of the ceacal microbiota in broilers treated with natural water additives. effect of natural water additives on broiler caecal micrbiota

This study aimed to investigate, via 16s rRNA amplicon sequencing, the effect of three natural water additive treatments; an organic acid (OA) blend, a medium chain fatty acid (MCFA) treatment, and an essential oil (EO) blend on the caecal microbiota of farm raised broilers. These three treatment groups were compared to both the general flock and a control group. It was found that all treatments had a considerable effect on the caecal microbiota, with the Firmicutes:Bacteroidetes ratio (F:B) and the other genera of interest varying between treatment groups. This study highlights the dybiosis that can occur with natural water additive treatment and further highlights the need for further research on the effect of broiler additives on the caecal microbiota.

本研究旨在通过16S rRNA扩增子测序（16s rRNA amplicon sequencing），探究三种天然饮水添加剂处理方案对农场养殖肉鸡盲肠微生物群的影响，具体包括有机酸（OA）复合制剂、中链脂肪酸（MCFA）处理剂以及精油（EO）复合制剂三种方案。本研究将上述三个处理组与常规鸡群及空白对照组进行对比分析。结果表明，所有处理方案均对肉鸡盲肠微生物群产生显著影响，不同处理组间的厚壁菌门：拟杆菌门比值（F:B）以及目标关注菌属均存在差异。本研究揭示了天然饮水添加剂处理可能引发的菌群失调问题，并进一步强调了开展肉鸡养殖添加剂对盲肠微生物群影响相关后续研究的必要性。