Galeruca daurica Assembly

To gain insight into the molecular mechanism of summer diapause, high-throughput sequencing was utilized in pre-diapause, diapause, and post-diapausing G. daurica . A total of 82292 unigenes were assembled using the Illumina HiSeq2500 platform. Through pairwise comparisons, 3099 and 81 significantly regulated transcripts were identified between pre-and diapause and diapause and post-diapause insects.

为阐明夏季滞育的分子机制，本研究应用高通量测序技术，对滞前期、滞育期及滞后期的G. daurica展开分析。依托Illumina HiSeq2500测序平台，共组装得到82292个单基因簇（unigenes）。通过两两比较分析，分别在滞前期与滞育期、滞育期与滞后期的样本中鉴定出3099个和81个显著差异表达转录本（transcripts）。