Sorted leukocyte populations from acute HIV-1 patients.. Homo sapiens

The aim of this study was to identify cell-type specific contributions to the acute HIV transcriptional signature identified in the CHAVI 001 acute 32 prospective cohort. Overall design: Four AHI patients and four healthy individuals were apheresed and different leukocyte populations were sorted by flowcytometry. Genome wide transcriptional profiling was done for AHI B cells (n=4), Healthy B cells (n=2) , AHI CD4 T cells (n=3), Healthy CD4 T cells (n=4), AHI CD8 T cells (n=4), Healthy CD8 T cells (n=2), AHI polymorphonuclear cells (n=2), Healthy polymorphonuclear cells (n=3), AHI monocytes (n=3), Healthy monocytes (n=4), AHI NK cells (n=3), Healthy NK cells (n=3) making a total of 37 samples.

本研究旨在明确细胞类型特异性对CHAVI 001纳入32名受试者的急性HIV前瞻性队列中鉴定出的急性HIV转录特征的贡献。实验整体设计如下：招募4名急性HIV感染者（Acute HIV Infection, AHI）与4名健康个体，进行血细胞单采术，随后通过流式细胞术分选出不同的白细胞亚群。对以下样本开展全基因组转录组谱分析：AHI患者B细胞（n=4）、健康对照B细胞（n=2）、AHI患者CD4 T细胞（n=3）、健康对照CD4 T细胞（n=4）、AHI患者CD8 T细胞（n=4）、健康对照CD8 T细胞（n=2）、AHI患者多形核白细胞（polymorphonuclear cells）（n=2）、健康对照多形核白细胞（n=3）、AHI患者单核细胞（n=3）、健康对照单核细胞（n=4）、AHI患者NK细胞（Natural Killer cells, NK）（n=3）、健康对照NK细胞（n=3），共计37份样本。