Mechanistic insights into anti-cancer properties of Curcumin and Andrographis in colorectal cancer

Colorectal cancer (CRC) remains the major leading cause of cancer-related deaths worldwide. The limitations of current chemotherapeutic drugs in CRC include their toxicity, side effects, and exorbitant costs. To assess these unmet needs in CRC treatment, several naturally occurring compounds, including Curcumin and Andrographis, have gained increasing attention due to their multi-targeted functionality and safety vs. conventional drugs. In the current study, we revealed that a combination of Curcumin and Andrographis exhibited superior anti-tumor effects by inhibiting cell proliferation, invasion, colony formation, and induction of apoptosis. Genomewide transcriptomic expression profiling analysis revealed that Curcumin and Andrographis activated the ferroptosis pathway. Overall design: Identify differentially expressed genes in Curcumin or Andrographis treated cells compared to non-treated cells

结直肠癌（Colorectal cancer, CRC）仍是全球范围内癌症相关死亡的主要致死原因。当前结直肠癌化疗药物存在毒性、不良反应及成本高昂等诸多局限。为评估结直肠癌治疗中尚未得到满足的临床需求，包括姜黄素（Curcumin）与穿心莲内酯（Andrographis）在内的多种天然化合物，凭借其多靶点功能特性及相较于传统药物的安全性优势，受到了越来越多的关注。本研究证实，姜黄素与穿心莲内酯联合使用可通过抑制细胞增殖、侵袭与集落形成，并诱导细胞凋亡，展现出更优异的抗肿瘤效果。全基因组转录组表达谱分析结果显示，姜黄素与穿心莲内酯可激活铁死亡（ferroptosis）通路。实验整体设计：对比经姜黄素或穿心莲内酯处理的细胞与未处理细胞，以鉴定差异表达基因。