Impact of Dietary Resistant Starch on the Human Gut Microbiome, Metaproteome, and Metabolome

We determined the impact of resistant starch on the gut microbiome and metabolic pathways in the gut, using a combination of “omics” approaches, including 16S rRNA gene sequencing, metaproteomics, and metabolomics. This multiomics approach captured changes in the abundance of specific bacterial species, proteins, and metabolites after a diet high in resistant starch (HRS), providing key insights into the influence of dietary interventions on the gut microbiome. The combined data showed that a high-RS diet caused an increase in the ratio of Firmicutes to Bacteroidetes, including increases in relative abundances of some specific members of the Firmicutes and concurrent increases in enzymatic pathways and metabolites involved in lipid metabolism in the gut.

本研究采用组学（omics）联用策略，涵盖16S rRNA基因测序（16S rRNA gene sequencing）、宏蛋白质组学（metaproteomics）与代谢组学（metabolomics），明确了抗性淀粉对肠道微生物组及肠道代谢通路的影响。该多组学方案捕捉了高抗性淀粉（HRS）膳食干预后，特定细菌物种、蛋白质及代谢物的丰度变化，为阐明膳食干预对肠道微生物组的调控作用提供了关键见解。联合分析数据显示，高抗性淀粉膳食可提升厚壁菌门（Firmicutes）与拟杆菌门（Bacteroidetes）的丰度比值，具体表现为部分厚壁菌门特定类群的相对丰度升高，同时肠道内参与脂质代谢的酶促通路及代谢物水平也同步上升。