Transcriptomic profile of circulating naïve, TCM, TEM and TEMRA T cells in donors from Sri Lanka previously exposed to dengue virus and healthy controls from San Diego [Cohort 2 & Cohort 3]

Dengue virus is responsible for 400 million human infections each year . The characterization of exact molecular components of immune response associated may help design more effective therapeutic interventions. In this study, we aimed to characterize the immune signature of T cells subtypes associated with previous exposure to dengue virus. Transcriptomic profiling using RNA sequencing was performed on naive, TCM, TEM and TEMRA CD4 T cells isolated from individuals with secondary dengue infections from Sri Lanka, an endemic area, as well as from dengue negative healthy controls. RNA-sequencing of sorted CD4 naïve, TCM, TEM and TEMRA T cells from cryopreserved PBMC of 5 subjects with secondary infection infection and 6 dengue virus negative healthy controls.

登革病毒（Dengue virus）每年可造成4亿人次人类感染。精准表征与之相关的免疫应答分子组分，或有助于开发更为高效的治疗干预策略。本研究旨在明确与既往登革病毒暴露相关的T细胞亚型免疫特征。研究人员对来自登革热流行地区斯里兰卡的继发性登革感染个体，以及登革病毒阴性健康对照者的初始型CD4 T细胞（naive CD4 T cells）、中枢记忆性T细胞（TCM）、效应记忆性T细胞（TEM）及终末分化效应记忆性T细胞（TEMRA）开展了RNA测序转录组分析。具体实验为：从5名继发性登革感染受试者与6名登革病毒阴性健康对照者的冻存外周血单个核细胞（PBMC）中分选得到上述四类CD4 T细胞，并对其进行RNA测序。