Single cell RNAseq data from Dlk1+ and Dlk1- aged murine hematopoietic stem cells. Single cell RNAseq data from Dlk1+ and Dlk1- aged murine hematopoietic stem cells

Quantification of gene expression noise on young versus aged bone marrow HSCs at single-cell resolution revealed Dlk1 increases both noise and expression levels upon aging. Dlk1, encoding a cell surface non-canonical Notch ligand, is partially expressed in the LT-HSC compartment, without generating clusters of individual subpopulations by conventional clustering analysis. We aimed to characterize the trascriptome of aged LT-HSC with positive and negative expression of Dlk1 cells. Dataset created as part of the publication: "VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cells" Overall design: LT-HSC (Lin-CD150+CD48-CD34-Kit+Sca1+) Dlk1+ and Dlk1- cells from bone marrow of 18 months old mice were sorted into 384-well plates. Cells processed for scRNAseq with a similar mCELseq2 protocol (Herman et al., 2018 Nat Methods, DOI: 10.1038/nmeth.4662)

以单细胞分辨率对年轻与老年骨髓造血干细胞（Hematopoietic Stem Cells, HSCs）的基因表达噪声进行定量分析，结果显示Dlk1在衰老过程中同时升高了基因表达噪声与基因表达水平。
Dlk1编码一种细胞表面非经典Notch配体，在长期造血干细胞（Long-term Hematopoietic Stem Cells, LT-HSCs）区室中呈部分表达状态，经常规聚类分析无法形成独立的亚群簇。
本研究旨在表征衰老LT-HSCs中Dlk1阳性与阴性细胞的转录组特征。
本数据集为发表论文"VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cells"的配套研究数据，该论文中文译名为《VarID2定量分析基因表达噪声动态并揭示衰老造血干细胞的功能异质性》。
整体实验设计：从18月龄小鼠骨髓中分选得到LT-HSCs（表型为Lin-CD150+CD48-CD34-Kit+Sca1+）中的Dlk1阳性与阴性细胞，接种至384孔板中。
采用与mCELseq2实验方案（Herman等，2018，《自然·方法学》，DOI: 10.1038/nmeth.4662）相似的流程对细胞进行单细胞RNA测序（single-cell RNA sequencing, scRNAseq）处理。